Biochemical Analysis of Protein SUMOylation
نویسندگان
چکیده
منابع مشابه
Global analysis of protein sumoylation in Saccharomyces cerevisiae.
Although the modification of cellular factors by SUMO is an essential process in Saccharomyces cerevisiae, the identities of the substrates remain largely unknown. Using a mass spectrometry-based approach, we have identified 271 new SUMO targets. These substrates play roles in a diverse set of biological processes and greatly expand the scope of SUMO regulation in eukaryotic cells. Transcriptio...
متن کاملAnalysis of Protein Biochemical Activities
Heng Zhu, Metin Bilgin, and Michael Snyder Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520; email: [email protected] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520; email: [email protected] Biological Sciences and Bioengineering, Sabanc University, Orhanli Tuzla Istanbul, 81474 ...
متن کاملIn vivo analysis of protein sumoylation induced by a viral protein: Detection of HCMV pp71-induced Daxx sumoylation.
Small ubiquitin-like modifiers (SUMOs) are covalently conjugated to target proteins to regulate numerous biological processes, including subcellular localization, protein-protein interactions, and transactivational activities. While the majority of identified SUMO targets are cellular proteins, SUMO modified viral proteins have also been identified. In addition, there are a growing number of ex...
متن کاملMechanisms, regulation and consequences of protein SUMOylation.
The post-translational modification SUMOylation is a major regulator of protein function that plays an important role in a wide range of cellular processes. SUMOylation involves the covalent attachment of a member of the SUMO (small ubiquitin-like modifier) family of proteins to lysine residues in specific target proteins via an enzymatic cascade analogous to, but distinct from, the ubiquitinat...
متن کاملTollip Is a Mediator of Protein Sumoylation
Tollip is an interactor of the interleukin-1 receptor involved in its activation. The endosomal turnover of ubiquitylated IL-1RI is also controlled by Tollip. Furthermore, together with Tom1, Tollip has a general role in endosomal protein traffic. This work shows that Tollip is involved in the sumoylation process. Using the yeast two-hybrid technique, we have isolated new Tollip partners includ...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2012
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb1029s99